Sentence examples for milk protein solution from inspiring English sources

The injection of an additional portion of milk protein solution led the SPR response on IgG to the return to its usual level.

Membranes were subsequently washed twice with the milk protein solution for 10 min each time, followed by incubation for 1.5 h with peroxidase-conjugated secondary antibodies, diluted in the blocking buffer.

Membranes were blocked for 1 h at room temperature with a milk protein solution [2.5%% (w/v) fat-free milk powder in 10%% phosphate-buffered saline] prior to incubation with primary antibodies to limit non-specific binding.

To prevent non-specific antibody binding, membrane sheets were blocked for 1 h with a milk protein solution consisting of 5% (w/v) non-fat dried skimmed milk powder in phosphate-buffered saline.

The membrane was blocked in 5% milk protein solution (Marvel, Lincs, UK) for 1 h at room temperature, incubated with primary rabbit polyclonal anti Kir7.1 antibody (1 200; Alomone Labs, Jerusalem) overnight at 4°C in blocking buffer and then incubated with polyclonal goat anti-rabbit HRP secondary antibody (1 100; Dako, Ely, UK).

Waste yak milk protein solutions were mixed and incubated with magnetic liposomes at 37 °C for 24 h so as to adsorb the active peptides out of solution.

In contrast to albumins, milk proteins (solution of the skim milk powder) demonstrated a high efficiency when being applied for the blocking procedure (Fig. 2, red) [26].

The cryo-sections were air-dried at room temperature, fixed in acetone for 5 min., washed for 15 min in Tris-buffered saline (TBS; 20 mM Tris-HCl, 500 mM NaCl, pH 7.4) and incubated for 1 hour with 1% purified milk protein blocking solution (Boehringer-Mannheim, Indianapolis, IN) in TBS to reduce background staining.

Sections were incubated with the primary antibody diluted 10-fold in a milk powder protein solution (5%, w/v) in phosphate buffered saline (PBS) for 1.5 h at room temperature.

After electrotransfer, blots were blocked for 30 min with 5%skim milk protein in antibody incubation solution (25 mM Tris-HCl, 0.15 M NaCl, 2.7 mM KCl, 0.05% Tween20 at pH7.4).

The surface was subsequently incubated with 10  μg/mL protein-G solution for 1.5 h, followed by a 2 h incubation with selectin chimera then blocked with 5% milk protein in PBS for 1 h.