Sentence examples for milk protein binding from inspiring English sources

The transcription factor, milk protein binding factor (MPBF/Stat5), is a member of the STAT family of signalling molecules which mediates prolactin signal transduction in lactating mammary gland by binding to GAS (gamma-interferon activation site) DNA elements.

After the PVDF membrane was incubated with 10 mM TBS with 1.0% Tween-20 and 10% dehydrated skim milk to block nonspecific protein binding, the membrane was incubated with primary antibodies overnight at 4 °C.

The membranes were incubated with 5% skim milk to block nonspecific protein binding and incubated with primary antibodies for p-p38 (1:1000, Cell Signaling), p-JNK (1 1000, Cell Signaling), α-tubulin (1 5000, Abcam), iNOS (1 1000, BD Pharmingen), and COX-2 (1:1000, BD Pharmingen) in 5% skim milk overnight.

Excretion of PFCs into milk may be accomplished by two ways that have been identified as transport mechanisms for chemical contaminants: binding to milk protein (protein content ~ 1 g/100 mL milk) or to the surface of fat (fat content ~ 4 g/100 mL milk) (Jensen and Slorach 1991).

Other studies have also reported that application of high pressure during enzymatic hydrolysis can effectively reduce the antigenicity and serum IgE-binding properties of milk protein hydrolysates (Beran et al. 2009; Chicón et al. 2008b; Peñas et al. 2006a, c).

The cytospin slides were then treated with 7% skimmed milk for 60 min to block nonspecific protein binding.

Proteins were then electrotransferred onto nitrocellulose membranes and the membranes were incubated in TBST buffer containing 5% nonfat dry milk for 1 hour to block nonspecific protein binding.

After blocking nonspecific protein binding with 10% dehydrated skim milk, the membrane was incubated with primary antibodies overnight at 4 °C.

Nonspecific protein binding was blocked using a 5% milk solution at 4°C overnight.

Nonspecific protein binding was blocked with 5%% skim milk in PBST (0.01 M, pH7.2 PBS containing 0.05 % Tween-20) at 37 °C for 30 min.

Nonspecific protein binding was blocked with 5% nonfat milk in PBS for 1 h and then incubated with primary antibodies at 1 : 1000 dilutions for 2 days.