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The colonies of E. coli BL21 containing only pET28a did not form any clear zones in the skim milk assay (data not shown).
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Data from testing the saliva IgG and IgA and also the milk and serum IgG with indirect SBV-specific ELISAs showed close agreement with the commercial serum and milk IgG assay data.
In fact milk 1,25(OH)2D3 was at or below the detection limits of the assay (data not shown) This was expected as the concentration of vitamin D metabolites is are low in milk compared to plasma [27], [34], and the 1,25(OH)2D3 produced by macrophages would have been diluted as milk accumulated in the cistern of the mammary gland.
The sensitivities of the newly developed tests were as follows: 96 % for the IgG serum assay and 94 % for the IgG milk assay and 85 % and 98 % for IgG and IgA in saliva tests, when compared with data generated by a commercial IgG assay.
Skim milk assay with CHA6.8ΔprtVΔVC1649 still showed residual protease activity (Fig. 1B).
All strains included in this study were also tested for protease activity in a skim milk assay.
The skim milk agar medium [ 36] containing 2% (w/v) skim milk (Baomanbio Co. Ltd ,Shanghai, China) was used in the skim milk assay to screen for protease-producing isolates.
Comet assay data.
Milk processing data were collected from 50 dairy plants nationwide.
1/Own estimation based on milk deliveries data from SHMPA and CREMPA.
Despite marked restriction of HIV-1 quantinies in milk, our data indicate intermixing of virus between blood and breast milk.
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