The Arabidopsis downy mildew resistant mutant collection and parent genotype eds1- 2 were a gift from Guido van den Ackerveken, Utrecht University, The Netherlands.
When you crawl under your sheets at night, you're tucking yourself away in a collection of pollen, mildew, fungus and maybe even some pet dander.
By using combination of microarray and SBS sequencing, a total of 125 putative long npcRNAs were identified in wheat leaves using strict criteria across a collection of more than 9700 powdery mildew and 6500 heat stress responsive sequences.
Throw away the vacuum bag or clean the collection container afterward to prevent re-infestation of mildew.
Like the wheat stem rust fungus, the wheat leaf rust fungus also appears to have a collection of proteins with the same characteristics as the powdery mildew Y/F/WxC-effector candidates (Additional file 6: Table S3).
Accessions maintained in the INRA collection that had Ren1 linked alleles were evaluated for powdery mildew resistance in an unsprayed greenhouse with susceptible controls and artificial inoculum in 2012.
In this study, we performed a genome-wide in silico screening of powdery mildew infection and heat stress responsive wheat transcripts in order to isolate a collection of long npcRNA genes.
The first was to evaluate a large collection of cultivated V. vinifera germplasm from Central Asia to identify additional powdery mildew resistant accessions using simple sequence repeats (SSR) markers linked to the powdery mildew resistance locus Ren1 on chromosome 13 [ 1, 2].
Other markers were selected, either due to their linkage to the known powdery mildew resistance loci Ren1, Run1, Run2 and Ren4 from previous studies or because they were used to manage the USDA National Clonal Germplasm Repository collection at Davis [ 2, 30, 41, 51].
Finally, it is clear that additional collections of Central Asian grape germplasm are needed to fully understand grape evolution, discover additional sources of mildew resistance, and ensure the survival of this historic and valuable resource.
