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Advantages of such a material are the circumvention of the need to remove the reducing agent after reaction, as well as milder reduction conditions and a concomitant lower risk of reducing the native disulfides.
Cellular infection with retroviruses expressing shRNA targeting c-Src gave a milder reduction in Src expression (70 75%) without affecting c-Yes level (Fig. 1B and Fig. S1).
Lastly, one might expect a milder reduction of iCa amplitudes in mdx muscle fibres, as a large fraction of DHPR should not be directly affected by the absence of dystrophin because (i) dystrophin is poorly expressed in the t-tubules as compared to DHPR and (ii) colocalization in wt fibres was about one third.
The milder reduction of mir-283 levels in mutant animals supports previous observations that mir-12 and mir-304 expression patterns show little correlation with that of mir-283.
To this end, we made use of an RNAi construct against the insulin receptor (InR) known to lead to incomplete receptor downregulation and a milder reduction in insulin signaling (Slaidina et al., 2009; Willecke et al., 2011).
We observed a significant, but milder reduction in nuclear abundance of wild-type DISC1 upon co-expression of 607F DISC1, and a change in the cytoplasmic distribution of wild-type DISC1 from punctate to diffuse.
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FoxO NR showed milder reductions in tissue size, therefore in subsequent experiments we used FoxO NK.
The term sleep restriction is commonly used for milder reductions of sleep duration continuing over weeks or longer.
Mild reduction of the disulfide backbone allowed complete release of pDNA from these polyplexes.
This is the reason why the monodispersed Ag/PANI/Fe3O4 nanoparticles can be obtained by the mild reduction reaction.
Though there was a mild reduction in glucose excretion in one of the patients, neither transplant was successful.
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