The wild type CF2 construct had a milder effect with this driver, resulting in IFM noticeably thinner than wild type, but intact from anterior to posterior, in contrast to the effects of the 88F-GAL4 driver.
We observed a significantly milder effect with mis-expression of Robo1, suggesting that Robo2's non-cell autonomous activity is not solely a consequence of Slit titration.
EA produced a milder effect compared with RA after incubation for 1, 6, or 24 hours.
This is in line with its described involvement in other inflammatory diseases, but with milder effect in the case of arthritis.
EA showed milder effects compared with RA, except for TNF- α.
Interestingly, several other miRNAs, such as miR-29a/b, miR-30a, miR-125a/b and miR-199 varied in a similar manner following stimulation, with a milder effect of IL-1β relative to TNF-α (Fig. 1B).
Stabilized oxychloro complex (SOC) (Purite, Bio-Cide International Inc., OK, USA) as an oxidant preservative also disrupts cellular membranes yet with a milder effect than BAK.
As seen in the EPICOR panel, we found LINE-1 hypomethylation also in Dutch cases as compared to controls, although with a milder effect (effect size ± se = −0.132 ± 0.042, 95%% CI −0.21;−0.05, P = 0.001).
Finally, loss-of-function mutations in the nucleic acid repair exonuclease TREX cause a severe immune-mediated neurodevelopmental disorder termed Aicardi-Goutieres syndrome, whereas mutations with a milder effect have been linked to chilblain lupus (Rice et al., 2007).
Most tools also tend to have low specificity, resulting in over-prediction of missense changes as deleterious and are not as reliable at predicting missense variants with a milder effect, Some of the more commonly used in-silico tools for missense variant interpretation in clinical laboratories include PolyPhen2, SIFT and MutationTaster.
Even with such a mild effect seen with CLA supplementation, there is still quite widespread interest in using CLA as a supplement [ 37].
