Sentence examples for mild trypsinization and from inspiring English sources

To differential passage, satellite cells were treated to a mild trypsinization and observed under a microscope until the round satellite cells were seen detaching from the plate.

At 72 hr intervals, each cell type was washed with PBS, subjected to mild trypsinization and diluted into 4 parts fresh growth medium.

Cells were stimulated with recombinant human CSF-1 or VEGF-A at different concentrations as indicated in figure legends, and after 48 hours they were washed with PBS, harvested by mild trypsinization, and counted with a hematocytometer.

24 h post-transfection, cells were either left adhered or detached by mild trypsinization and put into suspension on 1% agar coated tissue culture plates with 0.5% methylcellulose for an additional 24 or 48 hours, and analyzed by flow cytometry as described [19].

After the treatments, cells were collected by mild trypsinization and counted for cell numbers to construct a growth curve.

Detach the newly fused myotubes by mild trypsinization and replate them onto laminin-coated culture dishes or coverglasses.

In contrast, vehicle-treated cells exposed to the same de-adhesion conditions as their mifepristone-treated counterparts, were less sensitive to the mild trypsinization procedure, and depict normal morphology.

Then, microglial cells culture was prepared by mild trypsinization (0.05 % trypsin + 0.2 mM EDTA) and shaking.

Briefly, neurons were isolated by mild trypsinization (0.05%, w/v) and dissociated by passing them repeatedly through a constricted Pasteur pipette in a DNase solution (0.1%, w/v).

Briefly, cells were isolated by mild trypsinization (0.05% w/v) and dissociated by repeated passage through a constricted Pasteur pipette in a DNase solution (0.1% w/v).

ES cells were detached by mild trypsinization in EMFI medium and placed in suspension culture in dishes (Corning; 430591) coated with 0.1% agar (Gibco/BRL; M00391B).