Sentence examples for mild stripping from inspiring English sources

When necessary, membranes were subjected to mild stripping (stripping buffer: 200 mM glycine, 0,1 % SDS, 1 % Tween 20).

Mild stripping was performed using a buffer containing 1.5% glycin (w/v), 0.1% sodium dodecylsulfate and 1% (v/v) Tween 20 in aqua dest.

Membranes were stripped in mild stripping buffer (200 mM glycine, 0.1% (w/v) SDS, 1% (v/v) Tween-20, pH 2.2) for 45 min at 37°C before re-probing for the loading control anti-α-tubulin (Sigma, 1 10,000 dilution) for 1 hr at room temperature.

Membranes were stripped using mild stripping buffer (0.2 mM glycine, 0.003 mM SDS, 0.01% Tween-20, pH 2.2) and blocked with 2% BSA for 2 h at room temperature.

To detect phosphoeIF2α, blots were first probed with anti-phosphoeIF2α antibody, incubated with mild stripping buffer (0.1 M NaOH, 15') and then probed with anti-total eIF2α antibody.

Immunoblots were stripped using mild antibody stripping solution and re-probed with an anti-actin antibody (Santa Cruz Biotechnology, Inc).

To test multiple primary antibodies, membranes were stripped for 15 minutes at RT using ReBlot Plus Mild Antibody Stripping Solution (Millipore).

Before antibody staining the arrays were treated with ReBlot Plus Mild Antibody Stripping Solution (Chemicon, Temecula, CA) 1X for 15minutes at room temperature, rinsed 2 times for 5minutes in PBS 1X, and then blocked for 1 hour at room temperature in blocking solution (2gr I-Block - Tropix, Bedford, MA - and 0.1% Tween-20 in 1l of PBS 1X).

Pre-treatment of these cells with suramin or mild acid stripping failed to expose receptors which may have been occupied by endogenously produced ligand.

Mild steel strips were weighed after and before immersion time to record the weight difference.

The % of retention and % of recovery of the samples were calculated by the following equations: For impact resistance and scratch hardness studies, mild steel strips of 150 × 100 × 1.44 mm3were coated by the polymer nanocomposite solutions.