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There was only mild shaking in the resort city of Acapulco, according to reports.
At best, an airliner that encounters a pocket of swirling air experiences nothing more than a mild shaking.
The membrane fraction was homogenized in buffer I (pH 7.5) (10 mM HEPES-KOH, 5 mM MgCl2, 2% Triton X-100, 0.5% cholate, 10% glycerol and protease inhibitor cocktail) and incubated at 30 °C for 30 min with mild shaking.
Applying statistical tests to the results, an optimal procedure was developed that consisted of sample extraction by the solvent under mild shaking for 24 h and then removing the excessive solvent followed by drying the sample.
The reaction was carried out at 50°C in water bath with mild shaking.
The scaffold was then stained with 0.1%% of alizarin red solution and incubated for 30 min at mild shaking.
After hardening in the CaCl2 solution, the beads were rinsed with sterile water and then added to the 0.25% chitosan solution and kept under mild shaking for 30 minutes.
For instance, a giant quake off the coast or deep underground may produce only mild shaking in the closest city, while a small quake can wreak havoc if its fault breaks the surface where people live.
Pancreatic islets were then isolated by pancreatic duct injection of 500 U/ml of collagenase solution followed by digestion at 37°C for 40 min with mild shaking.
The C2C12 cells were stained by the DNA dye Hoechst 33342 (Sigma-Aldrich B-2261) at a final concentration of 11 µg/ml for 90 min at 37°C under mild shaking.
After being plated in the presence or absence of doxycycline for a period of 24 hours, mitotic cells were isolated by mild shaking and rocking for a period of 20 minutes to generate synchronized cultures.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com