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The splicing blocking morpholino (MO2) caused 22.5±1.1% and 52.6±1.2% severe as well as 23.7±1.5% and 18.7±1.4% mild deficient phenotypes at the concentration of 4 ng/embryo and 8 ng/embryo, respectively.
The translational blocking morpholino (MO1) caused 51.5±2.9% and 70.9±0.75% severe as well as 26.6±1.2% and 14.8±1.3% mild deficient phenotypes at the concentrations of 4 ng/embryo and 8 ng/embryo, respectively.
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Participant's vitamin D status will be defined by their blood 25OHD3 level, as: severely deficient <12.5 nmol/L; moderately deficient 12.5 25 nmol/L; mild deficiency 26 49 nmol/L; sufficient ≥50 nmol/L; and elevated >220 nmol/L.
75 subjects (74%) had vitamin D levels <50 nM and thus had at least mild deficiency, while 19 (19%) were severely deficient with levels <25 nM.
The first NCED gene (VP14) was cloned in maize by insertional mutagenesis, and exhibits mild ABA-deficient phenotypes due to gene redundancy (Tan et al., 1997).
The status of serum selenium has diverse effects on thyroid volume and goiter in a mild iodine-deficient or (borderline) iodine-sufficient area.
John et al. [ 12] found that when the intake of selenium was sufficient, there was no correlation between goiter and serum selenium levels even in a mild iodine-deficient area.
Mild G6PD-deficient subjects are recommended a dose of 0.75 mg base/kg one time per week for 8 weeks instead of the standard dose of 0.25 0.5 mg base/kg one time per day for 14 days.
We used immortal human breast MCF10A cells transformed by oncogenic Ras, grown for 4 weeks in a normal medium or in a mild folate-deficient medium (100 nM folate) and for an additional 2 weeks in a normal medium.
Cells were grown for 4 weeks in a normal medium or in a mild folate-deficient medium (100 nM) and then for 2 more weeks in a normal medium, to allow recovery of the cells from proliferation arrest due to the prolonged growth in folate-deficient conditions.
For this purpose, we injected Ras-transformed MCF10A cells, grown 4 weeks in a normal medium or in a mild folate-deficient medium (100 nM folate) and for an additional 2 weeks in a normal medium into (Atimic-Nu/Nu) nude mice.
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