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At the end of the study, rats were sacrificed by cervical dislocation under mild anesthesia and blood was taken by dorsal vena cava for various serological parameters.
At 28 days all groups animal sacrificed under using mild anesthesia and isolated the different organ (heart, liver, pancreas and liver) of the animal for histopathology.
At 28 day all the animal sacrificed under mild anesthesia and different organ (heart, liver, pancreas and kidney) of the animal was isolated for histopathology studies.
Later, animals were sacrificed by decapitation under mild anesthesia and liver was excised and stored at −80°C for further estimations.
At the end of the study 28 days all the groups animal were sacrificed under using mild anesthesia and isolated the different organ (heart, liver, pancreas and liver) of the animal for histopathology.
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Blood samples (0.2 ml) at specified time intervals i.e. 0.5, 1, 2, 3, 4, 6, 8, 12, 24, and 48 h were withdrawn from retro orbital choroid plexus (under mild anesthesia), collected in EDTA-tubes and finally the plasma was separated through centrifugation (rpm = 400, time = 10 min) and stored (− 40 °C), until further analysis through UPLC-ESI-Q-TOF MS/MS UPLC-ESI-Q-TOF MS/MSin-hoUPLC-ESI-Q-TOF MS/MS
After 28 days of treatment, blood sample was drawn from puncture the retro orbital under mild anesthesia condition, collected blood was centrifuged and examined for plasma glucose analysis by a GOD - POD method using the Glucose Estimation Kit (Span Diagnostic, India).
Another reason for low rates of initial screening, he said, is that many people are reluctant to undergo a procedure that requires fasting the day before, and then having a mild anesthesia while a doctor inserts a tube up the intestine to look for polyps.
After 8 hours of fasting and before euthanasia, blood was drawn from the retro-orbital vein of mice (mild anesthesia; 400 μl avertin, 200 mg/kg body weight) for VEGF and leptin determinations [ 24].
It should be a place where the temperature is mild (anesthesia renders them unable to regulate their body temperature), protects them from other animals, and is quiet and free of disturbance.
The mice were examined with an Inveon microCT (Siemen's Inc). in vivo under mild anesthesia (2.5% isoflurane).
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