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For this purpose, we performed single-cell force spectroscopy (SCFC) studies of PGCs and somatic endodermal cells from pre-migratory and migratory stages of development (Fig. 1A).
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Consistent with other reports in the literature, wortmannin significantly reduced migration of neutrophils into the tissue compartment, suggesting that the migratory stage of neutrophil response during inflammation may be an appropriate target for therapeutics.
However, the migratory stage of N. aberrans causes necrosis and lesions, suggesting that these G. pallida genes are not involved in suppression of host defences, and are probably involved in migration.
Neither of these genes is co-expressed with c-kit, however, and both have been used as specific diagnostic markers of xanthoblasts at migratory and post-migratory stages of chromatophore development [28], [33].
We quantified cellular adhesiveness of PGCs by single-cell force spectroscopy (Friedrichs et al., 2010), revealing a stronger attachment to their environment during pre-migratory stages of development in comparison to PGCs isolated from tailbud embryos.
Comparison of NC markers at the premigratory and migratory stages with that of Cxcr4 confirms that Xenopus NC cells are expressing Cxcr4 prior to and during migration.
Due to the difficulty of tracking passerines accurately over entire migratory stages, experimental studies have not been able to reveal the consequences of maintaining constant headings through the night.
QSOX1 is expressed during embryonic development in mouse and rat during key migratory stages [ 37].
The corresponding orthologues in N. aberrans are up regulated at J2 and migratory stages.
The position of R. similis and P. coffeae on each side of the bifurcation presumably implies that migratory endoparasitism is a prerequisite to sedentary endoparasitism and that the migratory parasitic stages of sedentary endoparasites have been lost in cyst and root-knot nematodes and yet retained as an important part of the life cycle in the "intermediate" genus Nacobbus.
In comparison to the pre-migratory stages, most of the PGCs isolated after the transition to active migration exhibit an altered cell morphology.
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