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For the wound-healing assay, cultured cells were wounded, photographed each hour, and quantified as the average linear migratory speed of the wound edges over 12∼16 hours.
In addition to G αq, CCR7 also used another pathway involving Rho/Pyk2/cofilin and presumably depended on G12/G13 to control the migratory speed of DCs [ 81].
The migratory speed of EA.hy926 cells treated with culture supernatants of Corin- siRNA H9c2 cardiomyoblasts was markedly decreased compared with that in NC- siRNA group using the wound healing assay (Fig. 6a).
Quantification revealed that in deep cortical layers the migratory speed of radially migrating 5-HT6R-shRNA1 TOM+ cells was significantly decreased versus scram-shRNA (Fig. 3D; supplementary material Movies 1 and 2).
Furthermore, an increase in the fibronectin matrix density leads to reductions in the migratory speed of Cx43 deficient CNCs, as shown via time lapse videomicroscopy (Xu et al. 2001).
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The Dunn chamber (Fig. 1A) was published in 1991 for the investigation of chemotaxis in fibroblasts, which migrate much more slowly at 0.42 1.25 µm/min, similar to the migratory speeds of cancer cells that move around 1 µm/min [19], [20].
This LFA-1 links to the cytoskeleton through α-actinin-1 and disruption at the level of integrin or actin results in loss of cell spreading and migratory speed due to a failure of attachment at the leading edge.
Altering the balance of adhesion affects migratory speed and indeed whether a cell moves at all [22].
Single cell nuclei were tracked over time, and migratory speed was calculated by dividing the length of the migration path by the total movie time.
Consequently, over 64% of APC pseudopods in the laser-treated or laser+OVA-treated groups had a migratory speed above 0.5 µm/min, whereas less than 10% of pseudopods in control and OVA alone groups showed a migratory speed above 0.5 µm/min (Figure 2D).
Further, we found that the highly invasive MDA-MB-231 cells are able to produce a certain amount of HGF, TGF- β or bFGF, which slightly influenced their migratory speed.
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