The effects of cryopreservation on mononuclear cell migration was analysed by comparing the migratory responses of freshly isolated and cryopreserved monocytes to various concentrations of the growth factors PlGF-1, VEGF-A, TGFβ1 and MCP-1 (Fig. 2).
To elucidate how gradient characteristics govern cellular response patterns, we here introduce an in vitro system allowing to track migratory responses of DCs to precisely controlled immobilized gradients of CCL21.
To identify specific molecular mediators of the proliferative and migratory responses of HSC to Cy/G-induced hematopoietic stress, we used DNA microarray technology to identify changes in gene expression associated with HSC mobilization.
Further, we demonstrated that the COMT Val158Met polymorphism, which alters COMT enzyme activity [3], [4], [5], predicts the adhesive and migratory responses of these cells to NRG1 in both normal subjects and in patients and that the group of patients with the high-activity Val allele was the most relatively impaired.
When the ligand of CCR7, CCL19 or CCL21, was added to the culture of CD3-engaged Treg cells with or without S1P, the migratory responses of WT Treg cells were highly enhanced compared with the responses of CCR7−/− Treg cells (Fig. 3B).
Migratory responses of cells to cellular guiding signals play important roles in regulating a wide range of physiological and pathological processes such as inflammation and autoimmune diseases [1], wound healing [2], [3], neuron guidance [4], [5], embryogenesis [6], and cancer metastasis [7], [8].
Galvanotaxis, the migratory response of cells in response to electrical stimulation, has been implicated in development and wound healing.
Impaired migratory response of bone marrow (BM -derived cells contriBM -derivedhibition of neovascellsization contributes toose cells [10].
The migratory response of mDCs treated for 2 days with LPS was clearly higher than that of the mDCs with low polySia content (Figure 2C).
To investigate this, the spontaneous and the CCL21 directed migratory response of DCs with or without polySia expression was measured in a transwell chemotaxis assay.
This suggests that BDNF, CA-Cdc42, and Rac1 may have influenced the reactivity and migratory response of the microglia and macrophages, which led to a decrease in inflammatory cytokine production.
