Collectively, our data indicate that MLK4 contributes to the acquisition of the mesenchymal phenotype and increased migratory potential of breast cancer cells through NF-κB-dependent mechanism.
Furthermore, we assessed the migration of high-MLK4 MDA-MB-436 TNBC cell line (Fig. 2a, b), and we found that migratory potential of the cells was significantly reduced upon MLK4 depletion (Fig. 4f).
Introduction of a small interfering RNA directed to ERRalpha into the highly aggressive breast carcinoma MDA-MB-231 cell line dramatically reduced the migratory potential of these cells.
Other studies have linked p53 to the control of the migratory potential of various cell types, including macrophages [62], [63].
The wound assay is frequently used to assess the effects of tested compounds on the migratory potential of adherent cells.
We have previously demonstrated that thyroid cells have the ability to increase migration in response to estrogen [21] and to determine if DIM can affect the migratory potential of these cells, in vitro migration assays were performed.
p-STAT3 has been implicated in microtubule remodelling, matrix metalloproteinase production [40], [41], and focal adhesion protein production [42], [41], leading to increased migratory potential of different cell types such as inflammatory and SCs.
Also, the changes in the links involving the adhesion molecules ITGA4, ITGB1 and ITGB7 confirm the role of IFN-ß treatment in decreasing the surface expression of adhesion molecules, which reduces the migratory potential of T cells [48].
Similar to earlier reports, the present study confirmed that depletion of uPAR inhibited matrigel invasion, adhesion to ECM components, and migratory potential of both non-irradiated and irradiated cells in vitro.
These results indicate that endogenous BTG2 expression contributes to the migratory potential of bladder cancer cells.
Fucoidan also decreased the migratory potential of Huh-7 cells by suppressing mesenchymal markers.
