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Expression of integrin subunit α6 has been linked to the acquisition of migratory potential in a wide variety of cell types in both pathological and physiological capacities.
We found a strong correlation between HIV-1 viremia, CCR7 expression, lower frequency in PBMC, and migratory potential in ex vivo assays.
We conclude that CCR7 upregulation on pDC of HIV-1 patients translates into an increased migratory potential in ex vivo assays.
This conclusion is also supported by our functional studies, which showed that pDC of viremic HIV-1 patients display increased migratory potential in in vitro assays.
As illustrated in Figure 2A, which shows both spontaneous motility (black columns) and motility in response to a chemoattractant (grey columns), parental cen3 fibroblasts, as well as early and mid cen3tel cells, had a very limited migratory potential; in contrast, tumorigenic cen3tel cells were clearly able to migrate through the porous membrane.
This loss of cell-stroma cross-talk is a well-known behavior of melanoma cells that acquired an increased migratory potential in an epithelial-mesenchymal transition.
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Wnt and Notch pathways have been implicated in EMT and migratory potential of cells in different types of tissues and cancer development [ 38, 39].
Future studies are needed to demonstrate whether this migratory potential indeed results in more efficient migration of DC into the lymph nodes and improved immune responses in cancer patients.
Radiation has been suggested to increase the invasive and migratory potential of GBM cells in vitro and in vivo [27], [28].
These include alterations in growth characteristics and in migratory potential, and altered expression of growth factors such as platelet-derived growth factor, insulin-like growth factor I and insulin-like growth factor II, transforming growth factor beta, hepatocyte growth factor and keratinocyte growth factor.
As shown in Figure 3C, Snail overexpression resulted in greater migratory potential, which could be abrogated by MSKE in ARCaP and LNCaP cells.
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