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One of the main migratory features of dermal fibroblasts is the extension of their filopodia along the collagen matrix [33], [34], [35].
The migratory features of Xenopus NCCs under 2D (Fig. 2a,b; supplementary material Movie 1) and 1D (Fig. 2c,d; supplementary material Movie 1) culture conditions were compared.
To confirm the anti-metastatic potential of EBP1 based on the relationship between EBP1 expression and clinicopathological parameters, wound-healing assays were first performed to examine the effect of EBP1 on the migratory features of ACC cells.
So far, no reports have associated VM with BNIP3 but based on our results, it is tempting to speculate that the down-modulation of the CD47 intracellular signaling, along with the significant changes in the adherent and migratory features of the BNIP3-silenced cancer cells, are crucially linked to the eradication of VM.
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Wild-type and heterozygous ICMs sustained a central cell mass and produced outgrowths of cells with refractile and migratory features characteristic of parietal endoderm.
The primary objective of this study was the development of a quantitative assay for cell migration, which could measure multiple migratory features, and would be compatible with high-throughput screening.
The rationale underlying this approach is that despite the obvious molecular complexity of the cell migration process, there might be "master genes" that could induce migratory features in a stationary cell.
The process of EMT involves the loss of cell-to-cell adhesions, loss of apicobasal cell polarity, and increment of migratory and invasive features of mesenchymal cells [ 48].
Our results showed that, while HIF-1 α was dispensable for cellular proliferation, functional and pharmacological inactivation of the factor lead to a significant reduction of migratory, invasive and adhesive features of human gastric cancer cells in vitro.
We have previously shown that Vegfa 120/120 mice have significantly fewer migrating GnRH neurons and demonstrated that VEGF164 signals through NRP1 to promote the survival of GN11 cells, which recapitulate many features of migratory GnRH neurons (Cariboni et al., 2011).
It is noteworthy that PC3 cells (a prostate cancer cell line) forcibly over-expressing osteopontin develop many features of migratory metastatic cells [ 7] but do not act as instigators in this model.
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