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In conclusion, for the first time, here we report that human PCs express chemokine receptors, present their profile, and demonstrate a dose-dependent migratory effect of distinct chemokines on these cells.
Both methods consistently demonstrated that PCs express receptors of all four chemokine subfamilies CC, CXC, CX3C, and C. Migration of PCs and a dose-dependent migratory effect of the chemokines CCL2 (MCCL25 CCL25 (TECK), CXCL8 (IL8), CXCL12 (SDF1α), and CXCL13 (but1), but not CCL22 (MDC) were demonstrated using a 96-multiwell chemotaxis assay.
Indeed, Matsuda and colleagues suggest that the migratory effect of Wnts can be blocked using a Rho kinase inhibitor [ 1].
The migratory effect of BMP2 on chondrosarcoma cell lines, however, suggests a role of BMP signaling in progression [ 14].
It is therefore not unlikely that a basal activity of these pathways may be of importance for the migratory effect of TPA.
However, as mentioned above, we could only reproduce the migratory effect of LTs in the present study and with the chosen experimental parameters.
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In addition, our results show clearly that p38α is a major protein responsible for the migratory effects of BMP-2 in mesenchymal cells.
To evaluate the proliferative and migratory effects of the newly found miR-203 target genes, RAN and RAPH1, we employed RNA interference to silence their expression in HEKn cells.
(4) To further investigate any migratory effects of individuals, we restricted follow-up time to when individuals were continuously resident in each county and to those who did not live in a county bordering England.
Comparison of the migratory effects of the chemokines on peripheral blood CD27- and CD27+ B cells in each individual showed that for each of the chemokines, the chemotactic response of CD27+ B cells was significantly greater than with CD27- B cells.
Finally, the anti-migratory effect of cordycepin against A549 cells was evaluated.
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