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Percent migratory cells was calculated using the following equation: = [(number of cells in bottom chamber)/(number of cells in input control)] × 100.

Our results suggest that the global RhoA activity in migratory cells was down-regulated in response to growth factor stimulation to a lesser extent than that in stationary cells.

The number of migratory cells was counted and the statistical data were presented in Figure 7D.

In case of PGCs isolated from tailbud stage embryos, however, the amount of migratory cells was increased to approximately 50%.

The nuclei were visualized by fluorescence microscopy, and the average number of migratory cells was determined from averaging four fields.

Images of three random fields (10× magnification) were captured from each membrane, and the number of migratory cells was counted.

Migratory cells were fixed, stained with 0.05% crystal violet, and counted in five randomly selected fields.

We isolated skin stimulator cells from 24 h transplanted allografts when most of donor migratory cells were gone19,20.

"We now know that these migratory cells are essential in the formation of HSCs, and we have described some of the molecular processes involved," Hollway said.

The evolution of the migratory cells is described by a diffusion reaction equation of non Fickian type deduced considering a mass conservation law with a non Fickian migratory mass flux.

Isolation of sub-populations of stationary and migratory cells were performed as described previously [36].