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Migratory cells in the bottom chamber were dissociated from the membrane, lysed, and quantified by adding CyQuant GR dye.
Our microarray data plus those of the migration assay obtained from primary astrocyte cultures strongly suggest that normal AA astrocytes in situ have the potential of becoming migratory cells in response to stress such elevated IOP as in glaucoma.
This model was commensurate with most migratory cells in the developing telencephalon being postmitotic; stem and progenitor cells reside in germinal zones and upon exiting the cell cycle their progeny migrate to final locations.
The number of invasive cells were obviously less than migratory cells in both cell lines.
The population of migratory cells in human articular cartilage tissue have been reported and termed as chondrogenic progenitor cells [ 57].
These experiments demonstrated that a large fraction of migratory cells in the RMS were proliferative (Fig. 1B-D) resembling their in vivo behavior.
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But why would migratory ability of cancer cells be advantageous in the first place, given that non-migratory cells in the primary tumor might have the advantage that resources could be spent on cell division rather than migration (Bernards and Weinberg 2002)?
Finally FLNabKD Jurkat cells exhibit a decrease in average speed which is, as in the case of ASB2α transfected cells, primarily due to the increased presence of non-migratory cells in the overall population (Fig. 9F left panel).
In vivo, this was used to show that the mobility of E-cadherin in cell membranes is lower in migratory cells than in stationary cells, and that inhibition of migration, through inhibition of Src, FAK or β1-integrin, reduces collective cell movement and increases E-cadherin membrane mobility (Serrels et al., 2009; Canel et al., 2010).
K6 is a marker of "active keratinocytes" that are proliferating, migratory cells identified in wound healing and other pathological conditions (Freedberg et al., 2001).
Several types of cell ECM junctions have been characterised in cultured cells (Dubash et al., 2009) including focal complexes formed at the leading edge of migratory cells, which in turn mature into larger more elongated focal adhesions (FA).
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