In vitro, the migratory capacity of isolated KO macrophages was significantly impaired on fibronectin, a substrate of MMP-14.
The effects of PGE2 on CCR7 expression have been correlated with increased migratory capacity of DC [2], suggesting that Ht31 may also be increasing the migratory capacity of DC.
For example, translocation of adenylate kinase (AK1) to focal contacts sites in MEFs induced migratory capacity of these cells [19].
The modified Boyden chamber assay demonstrated that the migratory capacity of EPCs was lost in doxorubicin-treated cells (Fig. 5).
Scratch assay using NOK-SI transfected with Rac1 siRNAs showed a strong inhibition of the migratory capacity of these cells (Figure 5 A-bottom).
However, this strategy appears to have limited therapeutic effect due to the limited longevity and migratory capacity of injected DCs [6] [10].
We tested expression of kinin receptors by flow cytometry and migratory capacity of circulating monocytes and progenitor cells towards bradykinin (BK) in transwell migration assays.
To this purpose we compared the migratory capacity of irradiated (15 Gy, single dose), vs. non-irradiated HUVEC in a scratch wound closure assay.
To substantiate a role for CD152 in mediating the migratory capacity of antigen-experienced T lymphocytes, we used T cells from CD152−/− and CD152+/+ mice.
In addition, it has been suggested that the migratory capacity of Treg cells is controlled by distinct signals from chemokines/chemokine receptors.
CCR7 and CCL21 contribute to the migratory capacity of the DC, but it is not completely understood what molecular requirements are involved.
