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We compared the migratory capabilities of SKOV-3 cells with Mock, RISC-free and claudin-1 siRNA-transfection.
Using a widely accepted planar migration assay we first explored whether the migratory capabilities of fetal and adult fibroblasts differed either at baseline or in response to growth factors implicated in wound healing.
These results showed that decreased GnT-IVa expression reduced the invasive and migratory capabilities of choriocarcinoma.
Id2 could facilitate the invasive and migratory capabilities of MCF-7 and SKOV-3 cells in a dose-dependent manner.
The distinct phenotypes of aASCs and scASCs were further demonstrated by the higher fat differentiation and migratory capabilities of scASCs.
All these data indicate that galectin-1 enhances the migratory capabilities of tumor astrocytes and, therefore, their biological aggressiveness.
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The general migratory capability of these five breast cancer cells has been well-studied [15], [22] [25].
Overexpression of calpastatin reduced the migratory capability of old cells, to the level of young cells infected with GFP alone (Fig. 7C).
As shown in Figure 2A, AQP1 knock down (siRNA AQP1-1) induced a significant reduction in the migratory capability of both cell lines.
Interestingly, Ci 1, the exogenous cell permeable calpain inhibitor, dramatically reduces the random migratory capability of both young and old cells (Fig. 7A).
To further validate the effect of DIM on the migratory capability of thyroid cells, a scratch wound assay was performed, which is a partial in vivo representation of metastatic phenotype [27].
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