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But activated CTLs showed a reduced migratory activity in the presence of norepinephrine and substance P which seems to be mediated via the Epac/Rap1 pathway as investigated by the use of specific cAMP analoga.
In these cells, the enhanced migratory activity in response to norepinephrine is based on the activation of the motor protein non-muscle myosin II [ 15], and is accompanied by changes in gene expression towards a metastatogenic phenotype [ 16].
This phenomenon was not initially observed in potato cyst nematode G. pallida J2s, which displayed no aberrant phenotype, or diminution of migratory activity in response to the same 0.1 mg/ml dsRNA 24 h soaks.
In particular, the combination FGF2+NT3+PDGF-AA resulted in the maintenance and enrichment of a proliferating oligodendroglial cell population displaying immature phenotype, limited self-renewal and increased migratory activity in vitro.
Our data showed that all the treatments trigger a 2 3 fold increase in migratory activity in both cell populations as compared to the basal condition (Fig. 5A, B).
In particular, the combination FGF2+NT3+PDGF-AA resulted in the maintenance and enrichment of an oligodendroglial cell population displaying immature phenotype (i.e., proliferation capacity and expression of PDGFRα, Olig1 and Sox10), limited self-renewal and increased migratory activity in vitro.
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Furthermore, developing cell migration and proliferation assays in which umbilical cord endothelial cells were evaluated for their ability to migrate and to proliferate, we observed an increase in proliferative and migratory activities in the presence of thymus extract [9], which are two relevant steps in angiogenesis, suggesting the angiogenic capacity of TAT.
DCP is a novel type of VEGF, having mitogenic and migratory activities in the angiogenesis of HCC.
Knockdown of ID3 in the medulloblastoma cell line resulted in decrease of cell viability and proliferation, enhanced apoptosis, and suppressed migratory activities in vitro.
Statistically significant changes were calculated from the mean migratory activities in the steady state phase using the unpaired and undirected Student's t test.
Statistically significant changes were calculated from the mean migratory activities in the steady state phase using the unpaired and undirected Student's t test; p-value lower than 0.05 was regarded as statistically significant.
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