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Converse to the inhibitory effect of overexpressing Tensin3 in normal cells, WM793 cells showed an increased migration when treated with specific siRNA for Tensin3 (Figure 6).
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In the migration experiments, the HN-7 cells seemed to detach from each other at the migration front when treated with HWF.
This suggests that δ-opioid receptor-OE keratinocytes have an innately higher level of phosphorylated PKCα/β, correlating with their enhanced migration even when treated with vehicle DMSO only.
Moreover, SMA-Ral reduced migration by 50 and 90% when treated with 5 and 10 μM, respectively).
Next, we evaluated the effect of tamoxifen on migration potential of MCF-7 and T47D cells and observed a marked reduction in migration of both of these cell lines when treated at a dose of 5 μM for 48 h (Fig. 2c).
Tb and TL cell migration was inhibited 26.2% and 31.4%, respectively, when treated with PDTC and was inhibited 24.8% and 34.4%, respectively, when treated with BAY 11-7085 (Fig. 4a).
A significant decrease in endothelial cell migration and vascularization was observed in the Matrigel plug assay in mice when treated intraperitoneally with 300 microg/day MMP-9 antisense for 21 days.
Prostate cancer cells, when treated with pomegranate juice, increased adhesion and decreased the migration.
The slight effects in migration observed might be explained by the reduced amount of secreted STIP1 when treated with siRNA.
Interestingly, despite the intense inhibition of cell migration by the miR-663 and miR-744 treatment, we did not observe any changes in cell migration when cells were treated with siRNA against eEF1A2 (Supplementary Figure 2).
Overexpression of Id1 increased migration (67.13 ± 3.82 compared to control of 36.33 ± 4.00) and similar effects were found when treating cells with both Id1 vector and cyclin D1 siRNA (67.24 ± 6.08).
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