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The silica occurs either as weakly cemented nodules or as hardpan and accumulates as a result of downward translocation (migration) when solubilized during weathering of the soil.
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When solubilized in decylmaltoside (DM), SaTRIC was crystallized in LCP in different lattices, including P63, P321 and R32, from various conditions37.
About half of the expressed PGM protein was present in inclusion bodies, but this protein was inactive when solubilized.
The Se-Met CpTRIC, when solubilized in β-octylmaltoside (OM), was crystallized in detergent micelles in the solution containing 38% PEG400, 0.1 M NaCl, 0.1 M CdCl2 and 0.1 M Tris-HCl (pH 8.5).
Stability can be engineered into IMPs by inserting stabilizing mutations, thereby generating proteins that can be successfully applied to biochemical and structural studies when solubilized in detergent micelles.
Here we apply cellular high-throughput encapsulation, solubilization and screening (CHESS) to the neurotensin receptor 1 (Nto1) to generate a variant that is stable in the apo-state when solubilized in detergents.
Xylanase fused to Zera was biologically active not only when solubilized from PBs but also in its insoluble form.
However, the structural stability parameters of SoPIP2 1 have not been examined neither when solubilized by detergents nor after the protein reconstitution into membranes subsequent to the heterologous expression.
FeCl3 solution results in a lower pH when solubilized in water.
It also forms a single oligomeric species when solubilized in digitonin (Fig. 1).
When solubilized in the bilayer fragment, Dz was 89 ± 1 nm and zeta potential, 26 ± 1 mV.
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