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As an alternative method to investigate migration, we performed a wound-healing assay.
To evaluate the role of claudin-1 in ovarian cancer cell migration, we performed a series of functional migration assays after manipulating claudin-1 expression in SKOV-3 cells using siRNA.
Due to fact that overexpression of Tensin3 in 293 cells markedly reduced cell migration, we performed siRNA knockdown experiments to investigate the role of endogenous Tensin3 in migration of a human primary cancer cell line.
To test the role of p38 in BMP-2-induced cell migration, we performed an in vitro migration assay using time-lapse imaging and analyzing the trajectory of cells for 16 hours (Movies S1 and Movies S2).
As the establishment of ordered cell polarity is a prerequisite for efficient cell migration, we performed migration assays using dHL-60 cells expressing EGFP-Syk or EGFP-Syk Y323F.
To investigate whether LAMA1 is required for hMSC migration we performed a wound healing experiment as described above but this time hMSC were cultured on either a laminin coated (20µg/well) or uncoated plate for 5 days under the same treatments described above (see Figure 3A).
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For comparison, the costs for the real migrations we performed are shown in Table 5.
To assess whether FGFR3-IIIc migrationmigration signals, we performed migration assays with SW480 and Caco2 cells after infection with dominant-negative KD3-IIIcv and Cv.
As a first step to identify loci having significant associations with three main categories of predictor variables (migration timing, morphology, and migration fate), we performed univariate analyses using a general linearized model (GLM) and a mixed linearized model (MLM) with TASSEL v. 5.0.8 (Bradbury et al. 2007).
For each migration rate, we performed daily growth-migration-dilution experiments with three initial relative abundances of AmpR to ChlR cells for each of two biological replicates, i.e., a total of six replicate pairs of co-cultures.
To further check the topology of the phylogenetic tree and the consistency of migration edges, we performed two independent runs of Treemix with eight migrations (Additional file 10).
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