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As adhesion is essential for cell migration, we next examined whether treatment with Sb supernatant could alter HCT-8/E11 cell attachment to ECM proteins.
Having established a role for Rac1 and IQGAP1 in [S1P]ext-induced cell migration, we next investigated the effect of down-regulation of SphK1 or SphK2 with siRNA on basal and [S1P]ext-induced redistribution of Rac1 and IQGAP1.
Since the migration results suggest that E-selectin plays a critical role in mediating breast cancer cell transendothelial migration, we next examined the interactions of breast cancer cells with a soluble recombinant protein of E-selectin extracellular domain (exE-selectin/Fc).
To characterize the role of CD44v4 in mediating breast cancer cell transendothelial migration, we next examined the effects of CD44v4 knockdown as well as removal of tumor cell surface sLex moieties on tumor cell adhesion to and migration across TNF-α pre-activated HUVEC monolayers.
To confirm this nuclear migration we next made direct observations of individual nuclei in live cells in real time.
Because miR-200c decreases migration, we next sought to determine the role of MSN in the ability of miR-200c to inhibit migration.
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Employing a transwell migration system, we next asked whether attraction of iNKT cells is mediated by CCR5 (Fig. 2D).
DOI: http://dx.doi.org/10.7554/eLife.03962.006 Since we observed dramatic Purkinje cell migration defects, we next examined the status of radial glial cells and Bergmann glia.
Using the co-culture model of cells migration described above, we next examined the effect of glial scar prepared from injured spinal cord on OECs migration 0, 1, 4, 7, 10 and 14 days after SCI.
As there is a large body of evidence linking PDGF receptor signaling with cell migration [ 36] we next set out to determine whether targeting PDGFR-β signaling could inhibit U2OS cell migration.
Having established a role for SphK1 and S1PL in S1P-mediated migration of HPAECs, we next studied the role of S1P1-3 on basal, [S1P]ext-, siRNAsiRNA - or 4-DP-induced cell migration.
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