Sentence examples for migration we generated from inspiring English sources

To determine the molecular mechanisms that contribute to cadherin-11-dependent cell migration, we generated cadherin-11 mutants.

To quantitatively explore the effect of migration we generated several data sets using the gradual separation scenario.

To test whether there were any cell-autonomous mechanisms influencing aberrant CNCC migration, we generated sham and ta chimeras, and quantified the percentage of chimeras in which donor CNCCs migrated away from the neural tube (Fig. 5A,A′).

To understand better the role of BCL2 in the mechanism of cell adhesion and migration, we generated doxycycline controlled expression of murine BCL2 in cultured NIH3T3 cells and a human BCL2 expressed in cultured MCF-7 human mammary adenocarcinoma cells.

Due to the potential confounding effects of FBS present in conditioned medium for stimulating T cell migration directly, we generated conditioned medium from osteoclasts cultured for 48 h in the absence of serum but supplemented with M-CSF and RANKL; conditions which did not adversely affect osteoclast viability as assessed by cellular morphology (data not shown).

To identify and characterize NFAT target genes that promote carcinoma cell invasive migration, we previously generated SUM-159PT breast cancer cells that inducibly express NFAT1 upon stimulation with dox [ 14].

To characterize the CD18-mediated signaling pathway downstream of Syk during PMN migration and infiltration, we generated dHL-60 cells transiently expressing an EGFP-tagged Syk mutant (EGFP-Syk Y323F), where the major binding site of the regulatory subunit p85 of the PI3K class IA was removed [13], [18].

To further determine whether RAC1 suppression is involved in the suppression of cell migration upon gefitinib treatment, we generated PC-9 cells overexpressing constitutive active forms of RAC1, RAC1G12V or RAC1Q61L.

To investigate the role of Snail on GMF-mediated cancer migration inhibition and EMT, we generated overexpressing Snail human colon cancer SW620 cells steadily expressing Snail cDNA and then confirmed the exogenous protein expression by immunoblot).

To track the migration of cells in 3D, we generated 3D cultures in which the distributions of cells in space were the same as those in Fig. 2 and 3 using MDA-MB-231 cells stably transfected with GFP and mTomato.

To further evaluate the correlation of RIP2 expression with migration or invasion in vitro, we generated RIP2 knockdown using two short-interfering RNAs (siRNAs), designated si-RIP2 #J and si-RIP2 #D, in the TBNC cell lines MDA-MB-231, Htb126, and SUM149PT.