The magnitude of HBMVEC migration was evaluated by counting the migrated cells in 3 random high-power (5x) microscope fields.
Metal migration was evaluated by the transfer coefficient (TC) and bioaccumulation factor (BAF).
The effect of different SPION concentrations (20, 40, and 80 μg/cm2) on VFF migration was evaluated using a scratch-wound assay.
Using cultured tenocytes, migration was evaluated by counting the number of initial cell outgrowth from the tendon explants and by transwell filter migration assay.
The ability to stimulate endothelial cell adhesion and migration was evaluated for Hyal, HyalS3.5 and their complexes with Cu II) and Zn II) ions.
Human lung fibroblasts were exposed to BAL fluids and cell migration was evaluated in collagen-coated Boyden chambers.
DC migration was evaluated using a 48-well microchemotaxis chamber (Neuroprobe, Pleasanton, CA) with 5-µm pore size polyvinylpyrrolidone polycarbonate filters (Neuroprobe) as previously described [61].
AM migration was evaluated in either 8 µm transwell cell culture insert or chemotaxis chamber as described previously [43] using Boyden chamber.
Human microvascular endothelial cells (HMEC-1 cells) were treated with vehicle (control), uric acid, 1, 7 dimethyl-uric acid (UA1) or 6, 8 dithio-uric acid (UA2) and cell migration was evaluated using a 24 well Transwell chamber chemoatraction assay.
