Exact(5)
To present long-term results of endoleak/endograft migration treatment by aortomonoiliac (AMI) endografting after failed endovascular aneurysm repair (EVAR) of infrarenal abdominal aortic aneurysms.
Because our experiments did not include a migration treatment we lack experimental support for this final phase; however, it seems likely that appropriate rates of migration among small populations would have the effect of causing more efficient peak shifts among small than large populations.
Using a 12 h wound-healing scratch assay to monitor cell migration, treatment with LPA was shown to result in 78% wound healing (P<0.005) in comparison with control cells in which 12% healing was achieved (P<0.05).
As with the analysis of sympatric resistance above, the relationship between phage infectivity and migration rate disappeared when the no migration treatment was excluded from the analysis (P > 0.1 for both linear and quadratic terms).
However the relationship between migration rate and sympatric resistance was likely to be solely due to the high rates of sympatric resistance at 0% migration; indeed, when the 0% migration treatment was excluded from the analysis, we found no significant effects of migration rate on sympatric infectivity (Fig. 1; P > 0.2 for linear and quadratic effects).
Similar(55)
The starting populations for the migration treatments were initially transferred without migration to allow some initial differentiation between populations.
Tubes from other migration treatments were chosen on the basis of shared founding populations: for example, replicate 1 in the no migration shared the same founding population as replicate 1 in the 0.01%, 0.1%, 1%, 10%, and 50% migration treatments, etc.
It is initially surprising that the 0%and50%0% migration treatments generated such different results: such high migration may simply have the effect of producing a single 3-fold larger population.
After six transfers, each population was used to seed six new replicate tubes, each of which was assigned to one of 6 migration treatments, resulting in a total of 108 (18 * 6) tubes.
We also addressed how migration rate affected global resistance of bacteria (i.e. the average resistance of bacteria from one treatment to phages from all other migration treatments) and global infectivity of phages (i.e. the average infectivity of phages from one treatment to bacteria from all other treatments), measuring infectivity and resistance across the range of migration regimes.
In addition, differences in ascertainment scheme significantly affect the relative PC1 score of admixed African lineages, under migration treatments a and c, as analyzed by ANOVA: (a) F = 5921, P = <0.0001; (b) F = 2.38, P = 0.09; and (c) F = 78.14, P = <0.0001.
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