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After migration, the cells at the bottom of the inserts were stained with DAPI and counted using fluorescence microscopy.
In mesenchymal migration, the cells attach to and pull on the matrix, through focal adhesion and cytoskeleton.
After migration, the cells at the bottom of the inserts were stained with 4′,6-diamidino-2-phenylindole (DAPI) and counted using fluorescence microscopy.
During cell migration, the cells interact with their surrounding environment by means of cell-cell interactions or cell-matrix interactions [43] [47].
Following migration, the cells were fixed with methanol for 10 min and stained with Giemsa for 30 min.
During migration, the cells actively extended pseudopodia to sense the direction of the wound site (Fig. 4A,C).
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Spontaneous cell migration allows the cells to forage and explore their surroundings by balancing random and directed migrations.
As a result, 3-D migration of the cells unquestionably limits cell spreading of the nanostructures.
Here we show that, in addition to causing cell death of hormone-refractory prostate cancer cells, PJ also increases cell adhesion and decreases cell migration of the cells that do not die.
The migration of the cells was monitored for 26 h by Live Cell Imaging (Figure 5B).
The rhuIFN β inhibited the migration of the cells in a dose-dependent manner.
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