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For cells in media maintained at pH 7.4, peak migration speed occurred at 1 µg/ml fibronectin, compared to a peak migration speed at 0.1 µg/ml fibronectin for cells in pH 6.5 media (Fig. 6C).
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The results (Fig. 11C) were essentially identical to those when cluster migration speed was changed while keeping cell migration speed normal (Fig. 11B).
Changing cell migration speeds above zero, while keeping cluster migration speed fixed at the normal value, failed to alter ALC diameters at any of the initial densities tested.
Further, the cell migration speed increased with an increase in the magnitude of the gradient.
There may also be a period of delayed migration in steelhead smolts prior to the start of seaward migration, which would explain the slower average migration speeds downstream.
(E ) Boxplot comparing the migration speeds in control vs MZ amotl2a −/− mutant pLLP.
When determining cell migration speeds a minimum of 30 haemocytes were measured from three embryos.
We note that the ligand density required for peak migration speed was substrate dependent, and occurred at higher fibronectin concentrations for cells plated on fibronectin-functionalized glass (Fig. S5).
Although we observed retraction events in vivo, these events were less severe and occurred less frequently during migration; therefore, the inhibition of ruffling enables lamellipodia to advance further and in doing so increase cell migration speed.
Silver migration speed was determined by stripping voltammetry.
Migration speed is adjustable.
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