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On a flat surface, migration speed decreases with matrix stiffness mainly due to an increased stability of focal adhesions.
Conversely, if the fiber density increases such that the mesh size becomes too small, the migration speed decreases and the cells may get trapped [ 9].
If the mesh size is too large, the migration speed decreases [ 9] due to a loss of cell-fiber attachment sites that are needed to either push or pull the cell body forward.
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Our cell migration experiments demonstrated that the fibronectin concentration required for maximum migration speed is decreased at acidic pH (Fig. 6C).
When sediments pass a meander bend of a river channel with a pool, the migration speed of sediments decreases greatly as they enter the pool, and it then increases as they leave (Kleinhans et al. 2002); this resembles the behavior of cars entering and leaving traffic jams.
This activity is thought to facilitate actin polymerisation, enhancing the generation of actin-rich protrusions; however, in these cells exuberant Ena/VASP activity actually decreases migration speed because the protrusions formed are unstable and tend to be lost as membrane ruffles (Bear et al., 2000; Bear et al., 2002).
In addition, we report that this mutation induced decreased migration speed but increased invasive properties.
The fact that Ena overexpression increased the rate of two distinct examples of directed migration in vivo whereas inactivation decreased migration speed is in stark contrast to similar experiments conducted with fibroblasts in vitro.
Myo10 knockdown ablated filopodia and decreased 2D migration speed.
Myo9b knockdown increased stress fiber formation, decreased 2D migration speed, and increased directional persistence.
Interestingly, we found dramatic differences in cell polarity associated with the decrease in migration speed.
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