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This recapitulated in reverse the normal migration sequence and showed good qualitative agreement between theory and experiments.
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They concluded that it was most plausible that these migration sequences were due to the propagation of slow slips.
Movie S1 shows typical migration sequences of wild type and THP-1 cells expressing the double tagged α2AAR-YFP/CFP in response to UK 14'304 and CCL2.
Samples that showed variant migration bands were sequenced by direct sequencing using the ABI BigDye cycle sequencing kit with dye-labelled terminators.
Cellular migration involves a sequence of well-documented events [ 46- 49].
It has been recognized recently as a reliable biological marker of host-pathogen co-evolution and ancient human migration based on sequence variation in select gene loci.
Any fragments showing migration shifts were sequenced directly from the PCR products using the ABI Ready Reaction Dye Terminator Cycle Sequencing Kit and an ABI3100 Prism sequencer and analysed using the Sequence Navigator software.
While we still cannot preclude that aspirations havechanged already before the migration experience, the sequencing is clearer in thisset-up.
Cloning the target sequences into bacteria and sequencing different clones using the Sanger approach, so as to recover the different variants, may be required to further analyze relationships between migration patterns and sequences e.g. [ 25].
We demonstrated that the migration and adhesion sequences of breast cancer cells, induced by SDF-1α gradients, involves successively the activation and inactivation of RhoA and an increased expression of Rac1 through the gradient.
Recent coalescent methods like IM model (Isolation with Migration) based on sequencing loci from different inherited systems can also help to address these questions by estimating the extent of genetic divergence and revealing demographic dynamics by distributional changes, which are applied to within species or between recently divergent taxa [ 12, 13].
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