With the fall migration, blood samples were taken from a group of six storks, consisting of four fledglings and two older birds.
To determine if the FE-Pro complexes were well-retained and encapsulated within NSCs after implantation into the mouse brain and subsequent tumor-tropic migration, the brain samples were analyzed with TEM and immunohistochemistry.
Migration rates between population samples were all of the same order, and no preferential directionality of the migrants was observed.
Because the BV GP64 protein has a similar migration with SEAP, supernatant samples were also subjected to ultracentrifugation at 80,520 × g for 60 min to pellet BV particles, as a control.
For CS and HS, the migration positions of standards and samples were similar, but for DS the slight difference in migration suggested composition or structural differences in the arterial DS compared to the standard.
To control for differences in gel migration, exposure time, antibody incubation etc, samples were run on the same gels and transferred to the same PVDF membranes (Amersham, UK).
Estimates of pairwise migration rates (Nm) between adjacent EPR samples were similar, ranging from 2.0 to 5.3 after excluding the three southern SEPR samples for which F ST ≈ 0. Nonetheless, the existence of regional alleles (blue and red in Figure 1D) suggests that dispersal across the Equator might not have achieved equilibrium with genetic drift (discussed below).
Blood samples were taken during both autumn and spring migration for each of the following temporal subsets: 1985/86, 1988/89, and 2006/07, with additional samples from spring 2001 migration period (see Table 1 for sample sizes).
All samples were analyzed by the modified A. alata mesocercariae migration technique (3 ).
