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Exact(6)
Changes in the migration position of the BSA bands in the aliquots were compared.
We identified the migration position of acetyl-P (Fig. 3, panels B-D, arrow) using three different approaches.
Consequently the migration position of diglycosylated band in PrPres-2 coincides with that of the monoglycosylated band of population PrPres-1 [ 20, 30].
Search parameters were set on mass tolerance <0.1, p I and Mr as compared with the migration position of a spot in the 2D gel, and ' homo sapiens' was selected for species search.
The migration position of radiolabeled spots (Rf; ratio of spot migration:mobile phase migration) was visualized using a phosphorimager and were 0.65 (GTP) and 0.81 (GDP), matching equivalent values calculated from Tee et al. (51).
However, we cannot fully exclude the presence of other globins, because the migration position of intact chains of these subunits is outside the pH 4 7 gradient range, and the concentration of proteolytic cleavage products with pI values smaller than 7 could be too low to be detectable by protein gel staining or mass-spectrometric analysis.
Similar(54)
We emphasize image warping and related methods that are able to overcome the difficulties that are due to varying migration positions of spots between gels.
Commercials standards were used to identify the migration positions of the GAG: dermatan sulfate (DS), chondroitin sulfate (CS), and heparan sulfate (HS).
For CS and HS, the migration positions of standards and samples were similar, but for DS the slight difference in migration suggested composition or structural differences in the arterial DS compared to the standard.
The biased filopodia orientation was followed by cell polarization (blue arrow in Figure 4D) as manifested by the elongation of the cell along the axis of migration, positioning of the golgi to the back of the cell and migration towards the chemokine source (Video 6, Cxcl12a transplant, with snapshots presented in Figure 4B of a single cell representing 15/17 cells that showed this response).
Rudd and coworkers have established a database (GlycoBase and autoGU) which allows the structural assignment of 2-AB-labeled oligosaccharides on the basis of migration positions on a TSK-amide-80 column.
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