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Results display influence of ECM collagen architecture and collagen fiber spatial direction on migration of stem cells.
Migration of stem cells to the affected site and promotion of vascularization are essential for tissue engineering therapy, including bone regeneration.
To further confirm the inhibitory effect of SERPINB2 on the migration of stem cells, western blotting was used to evaluate the levels of MMP-2, which plays a key role in regulating MSC migration.
The proliferation, differentiation, and migration of stem cells are crucial during tissue regeneration, and the Hippo pathway has been shown as an important regulator in stem cell function.
Our results suggest that intravenous application of DF-MSC results in migration of stem cells to damaged ileoceceal tissue where they can function and protect the integrity of ileal tissue.
Among the currently available surgical approaches, bone marrow stimulation procedures aim at favoring the healing process in the lesion site, taking advantage of the migration of stem cells from the subchondral bone with minimal invasiveness and limited costs, even though paired with well-known limitations (Orth et al. [2012]; Kon et al. [2011b]).
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As seen in other RSA studies, in both groups most of the migration of the stem occurred between the stem and the cement mantle, with little movement between the cement and bone.
A transwell migration assay demonstrated the inhibitory effect of toxic materials on the migration ability of stem cells in vitro (Fig. 1c).
A transwell migration assay demonstrated the inhibitory effect of toxic exposure on the migration ability of stem cells in vivo (Fig. 2c).
The outcome variables were migration of the stem measured with RSA, changes in bone mineral density (BMD) in 7 Gruen zones around the stem, the occurrence of periprosthetic fractures and other hip-related complications, and clinical outcome.
Physiological migration of hematopoietic stem and progenitor cells.
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