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Initial migration of RAW 264.7-GFP at the interface with the collagen drop was imaged at 20X (NA = 0.75) for 12 hrs at 4 frames/hr using a Nikon C1-Si inverted confocal microscope (Nikon Instruments Inc., Melville, NY) equipped with PMT detectors and lasers appropriate for GFP (488 nm) and DsRed (561 nm).
Given that the activation of these signaling pathways is often detected following membrane receptor(s) engagement and that C1P was shown to stimulate migration of RAW 264.7 macrophages in a PTx-sensitive manner [15], we examined whether the mitogenic action of C1P could be mediated by a Gi protein-coupled receptor.
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These findings are interesting in light of the fact that we did not detect changes in the migration behavior of RAW 264.7 cells when co-cultured directly with CT26 cells.
IQGAP1 also binds to actin filament nucleator, Diaphanous-related formin (Dia1), and thus localizing Dia1 at the actin-rich phagocytic cup, which is required for migration and phagocytosis of RAW macrophage [17].
Directed migration may be important to the fusion of RAW 264.7 cells to multinucleated, differentiated osteoclasts, and we hypothesized that IL-8 may play a role in this process.
The occurrence of exotic raw material confirms migration of hunter groups in the late Younger Dryas in the latitudinal direction.
Interestingly, in contrast to the co-culture system where RAW 264.7 did not exhibit an increase in migration distance or displacement, the gradient of chemotactic factors in the Boyden chamber was sufficiently steep to elicit robust chemotaxis of RAW 264.7 macrophages.
A man, he says, of raw courage, raw integrity.
The idea of raw fish repulsed me.
He smelled of raw oysters and seaweed.
And there are questions of raw power.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com