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In all cell line tested, Mitostatin over-expression inhibited the ability of cells to migrate (Figure 3A) indicating that Mitostatin negatively regulates migration of prostate cancer cells.
Uysal-Onganer, P. et al. Wnt-11 promotes neuroendocrine-like differentiation, survival and migration of prostate cancer cells.
Frigo, D. E. et al. CaM kinase kinase beta-mediated activation of the growth regulatory kinase AMPK is required for androgen-dependent migration of prostate cancer cells.
Here, we show that a stretch of proline residues located within the N-terminus of androgen receptor (AR) is a bona fide coregulator binding surface, the disruption of which reduces the androgen-dependent proliferation and migration of prostate cancer (PCa) cells.
DBP-maf also had the ability to inhibit migration of prostate cancer cells in vitro.
Next, we determined the ability of Mitostatin to inhibit migration of prostate cancer cells using an in vitro "wound-healing" motility assay [21].
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In contrast, the migration activity of prostate cancer cells significant increases when exposed to the neurotransmitter dopamine and neuropeptide substance P up to 25%.
Cell migration is a major determinant of cell invasion11, and KBU2046 inhibited the migration of human prostate, breast, colon, and lung cancer cells (Fig. 1c).
CTN06 also impeded the migration of human prostate cancer cells based on a 'wound healing' assay.
We therefore investigated whether MP1 influences ERK and/or PAK signaling during migration of DU145 prostate cancer cells.
For example, in hepatocellular carcinoma, ectopic overexpression of miR-224 significantly down-regulated HOXD10 expression and promoted cell migration and invasion [ 12], whereas inhibition of miR-224 expression enhanced cell migration and invasion of prostate cancer cells through direct regulation of oncogenic TPD52 [ 29].
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