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This approach has proven especially powerful in the hematopoietic system, where it has been possible to monitor the generation, expansion, maturation, and migration of primitive erythroid cells, macrophages, and megakaryocytes during embryogenesis at unprecedented resolution.
These results suggest that Prtg may have roles in migration of primitive mesoderm cells, neuronal differentiation and tooth germ formation.
Such a strategy might have facilitated the migration of primitive cells, which had not yet developed means for supporting motility by specifically adhering to their environment.
We first evaluated the migration of primitive myeloid cells in spib morphants by taking advantage of the range of phenotypes obtained with spibe1i110ng and spibATG10ng morphants.
This allowed us to observe the migration of primitive myeloid cells away from the aVBI, using time-lapse fluorescence video microscopy.
Recent evidence suggests that SDF-1 and S1P work synergistically to facilitate migration of primitive murine progenitor cells out of the BM [ 59].
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In the current experiments, the migration of primitive-streak cells transfected with green fluorescent protein (GFP) fusion proteins with PTEN and several PTEN mutants was followed over time by using fluorescence time-lapse microscopy, allowing a detailed characterization of the migration behavior of these cells and the demonstration that PTEN has two separable mechanism of action in this assay.
Additionally, during embryonic development, knockdown of the puf-A gene led to a reduction in the number of PGCs and their abnormal migration, suggesting that Puf-A is involved in the maintenance and migration of these primitive germ cells.
For the evaluation of migration of cardiac primitive cells in the presence of biomatrix, cells were grown to confluence, and a thin scratch was introduced on culture plates with a pipette tip, producing a cell-free zone [ 11].
Migration of cardiac primitive cells) was the fastest on Bm-N; in the presence of Bm-P, it was similar to that of control but significantly slower when compared with the speed of migration on Bm-N (n = 9, P < 0.05).
Moreover, migration of these primitive cell populations to the grafted heart resulted in their loss of stem-cell markers, active proliferation, and acquisition of the mature phenotype followed by cell colonization and de novo formation of myocytes, coronary arterioles, and capillaries [ 2].
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