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Cnot2 involves in the migration of lymph cells [25].
Migration of lymph node cells was increased in response to CXCL10 (chemotactic index was 1.91 ± 0.45).
Inguinal lymph node cells of CIM were harvested and the migration of lymph node cells was evaluated using a Costar Transwell system (24-well, 5-μm pore size membrane; Corning Costar, Cambridge, MA, USA).
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Absence of DOCK8 results in failure of DC migration to lymph nodes and defective CD4+ T-cell priming [ 77].
This finding is consistent with the induction of DC migration to lymph nodes upon activation.
Maturation of DC is necessary to enable migration to lymph nodes and activation of T cells.
The activated epithelium releases inflammatory cytokines that enhance dendritic cell migration to lymph nodes, recruitment of innate immune and Th2 cells to the airways, and activation of Th2 cells [ 3, 4].
Our results further support the hypothesis that the beneficial effect of sleep on adaptive immunity is at least partially mediated by sleep-dependent increases in MR signaling, with a consequent facilitation of T-cell migration to lymph nodes.
In conclusion, we successfully monitored the proliferation of DCs and their migration to lymph nodes in live mice by performing 18F-TFB PET imaging of the NIS gene and optical imaging of the effluc gene.
Since maturing DCs express the CCL19 ligand CCR7 that directs migration of DC towards lymph nodes, we analysed CCR7 expression after SFA treatment (Figure 7 B, C).
Next, we sought to determine whether there might be underlying mechanisms that compensate the migration of pDC to lymph nodes in HIV-1 patients, thereby offsetting in part accumulation of these cells.
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