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In order to observe some of the cellular events, more specific migration of leucocytes at the site of injury, expression of the marker CD45 was analysed (Figure 8 a-f).
Intercellular adhesion molecule-1 (ICAM-1) binds to lymphocyte function-associated antigen-1 (LFA-1) and macrophage 1 antigen (Mac-1) on immune cells, and is involved in adhesion and migration of leucocytes in an inflammatory environment.
Migration of leucocytes from blood vessels to the flamed sites is a fundamental feature of inflammation.
Transendothelial migration of leucocytes across the vessel wall into the tissue follows cellular adhesion.
CD18 /– mice show a cell-autonomous defect of migration of leucocytes to the site of inflammation.
This further increases the adhesion and finally migration of leucocytes and lymphocytes from the blood into the inflamed joints [ 11].
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Platelet endothelial cell adhesion molecule (PECAM) also binds cells together but in addition is required for promoting the migration of leucocyte migration across the endothelium [ 24].
Solubilized antigens, similarly prepared from normal leucocytes, showed inhibition of migration of remission leucocytes to the extent of 15% only.
The same batch of CML antigens inhibited migration of normal leucocytes at the level of 22-222-2%
No enhancement of migration of remission leucocytes was seen with CML antigens.
Cytokine-mediated activation of the endothelium facilitates migration of autoreactive leucocytes into peripheral nerves in EAN (Stoll et al., 1993).
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