Exact(1)
This may be due to a delay in the initiation of migration of "leading edge" mesoderm towards the animal pole ("anterior" of the embryo).
Similar(59)
Regulation of leading edge assembly and cell migration by factors downstream of small GTPases and PI3Ks is also accomplished by activation of numerous kinases, such as ROCK, PAK or LIM Kinase-1 (LIMK1) [6].
We also show that BMP-2 activation of p38/MK2 leads to phosphorylation of Hsp25 and that this phosphorylation and possibly other actin-capping proteins is relevant for BMP-2 induction of leading edge formation and cell migration.
We see that the effect of leading edge saturation is to establish a new effective leading edge for new captures.
The inner surface of the blastocoel roof is ready for the directional migration of the leading edge along the surface of fibronectin substrate (Nakatsuji et al. 1982; Boucaut et al. 1984; Winklbauer & Nagel 1991; Johnson et al. 1993), and the migration was observed in vivo and in vitro (Hara et al. 2013; Moosmann et al. 2013).
Blade bowing induces variation of the inlet CF, thus changes the radial pressure gradient and leads to flow migration before leading edge (LE) in the cascades.
But the anterior migration of the leading edge after ACE is definitely observed, as many researchers have reported (Nakatsuji et al. 1982; Boucaut et al. 1984; Winklbauer & Nagel 1991; Johnson et al. 1993).
The proportion of cells polarised in the direction of migration did not differ significantly between genotypes (Fig. 4D,E), nor did the distance migrated by leading edge NC cells from the central explant (Fig. 4F).
All experiments were repeated at least three times and the measurements were made using tools in Adobe Photoshop CS4 To monitor the migration of lateral hypoblast leading edge cells (marginal deep cells), the embryos at 75% epiboly stage were dechorionated with 0.01 mg/mL protease (Sigma, St . Louis MO) and mounted in 0.8% low-melting agarose (Amresco, Solon, OH).
Thus LFA-1-mediated migration of T lymphocytes requires Rap2-expressing vesicles to recycle the extended form of LFA-1 that we have previously found to control migration at the leading edge.
Cell migration involves formation of a leading edge in the direction of migration and adhesion points from which tension is generated to move the cell body forward.
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