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In vivo, VEGF controls the correct migration of facial branchiomotor neurons in the developing hindbrain and stimulates the proliferation of neural stem cells in enriched environments and after cerebral ischemia.
We found that the early trajectories of the migration of facial and accessory abducens neurons were different with adjacent paths radially followed by distinct paths dorsally [ 13].
Components of the planar cell polarity (PCP) pathway are required for the caudal tangential migration of facial branchiomotor (FBM) neurons, but how PCP signaling regulates this migration is not understood.
pk function is required for the migration of facial branchiomotor neurons in zebrafish (Carreira-Barbosa et al., 2003; Bingham et al., 2010; Mapp et al., 2010; Mapp et al., 2011).
A further zebrafish allele of Spt5 (fh20) was isolated from a genetic screen to identify genes that control the posterior migration of facial branchiomotor neurons in the zebrafish hindbrain 36.
To identify novel genes required for the tangential caudal migration of facial branchiomotor (FBM) neurons, we conducted a forward genetic screen using Tg isl1 GFP rw0 transgenic zebrafish, which express GFP in branchiomotor neurons (Fig. 1A) (Higashijima et al., 2000).
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According to the literature, Nrp1 -/- mutants have a defect in which the migration of the facial motor neurons is disrupted.
Fz3 and Celsr1-Celsr3 alsoalso required for caudal and tangential migration of VIIth cranial (facial branchiomotor) nerve neurons within the developing brainstem (Qu et al., 2010).
The involvement of PCP genes in neuronal migration comes from the study of facial branchiomotor (FBM) neurons in the segmented hindbrain of vertebrates.
In the case of facial branchiomotoneuron migration, cdh2 and Tag1 seem to play a crucial role (Wanner and Prince, 2013; Sittaramane et al., 2009); however, these molecules have no influence on trigeminal neuronal migration.
Third, the observation that VEGF120 cannot compensate for VEGF164 in NRP1-dependent neurons has precedence in our previous studies of facial branchiomotor neuron migration and retinal ganglion axon guidance (Schwarz et al., 2004; Erskine et al., 2011).
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