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The nested collagen matrix model is a relatively easy, rapid and quantitative method to measure migration of cell populations.
Previous work has focused primarily on remodeling and contraction of collagen matrices by fibroblasts, and there has been little research on migration of cell populations within the matrix.
After GNP-NKCT1 treatment, cell cycle arrest as well as programmed cell death occurred via apoptosis and for this reason migration of cell became very less after wound healing.
Migration of cell from the edge of the injured monolayer was measured using Laser Capture Microdissection Microscope (LCMM, Zeiss, Germany) along with Palm Robo software at 25 different positions.
Our recent studies demonstrate that proliferation and migration of cell lines stably expressing UCH L1 siRNAs is reduced, and the sensitivity to apoptotic agents was increased [38].
The transient existence of this layer could be linked to the protracted proliferation and tangential migration of cell precursors in subpial position, which are restricted to the EGL in rodents [60] but find a permissive environment for longer time in lagomorphs.
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In addition, transwell migration assay confirmed significantly reduced migration of cells overexpressing miR-215-5p miR-215-5p miR-215-5p
The migration of cells was analyzed using scratch wound migration assay.
The migration of cells was examined using a Transwell chamber.
This triggered migration of cells out of the neurosphere (Fig. 4h; Supplementary Movies 12 and 13).
Inflammation accompanies massive migration of cells and cytokines, which not only requires, but also elicits ECM remodeling32.
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