As shown in Fig. 3, panel A, sHLA-G treatment significantly dampened the chemotaxis of CD4+ T cells towards i) two ligands of CCR2, i.e. CCL2 (migration index 2,9 vs 0,7, p = 0.033) and CCL8 (migration index 2 vs 0, p = 0.048) and ii) two ligands of CXCR3, i.e. CXCL10 (migration index 13,77 vs 2,21, p<0,0001) and CXCL11 (migration index 47,40 vs 14,55, p = 0,012).
Moreover, the hydrogen index (HI), oxygen index (OI), production index (PI), and migration index (S 1/TOC) were calculated.
In particular, the association between disease prevalence and reported characteristics, such as demographic indices, lifestyles, mental health state, and migration index, will be investigated.
Impact of 1400 W (D) and L-NMMA (E) at micromolar concentrations on the migration index in MDA-MB-231 and SUM159 cells.
The mean ± standard deviations (SD) were recorded for each condition, and the migration index was calculated based on the control involving random migration.
Results are expressed as migration index: number of migrating neutrophils in a defined condition divided by the number of migrating neutrophils toward medium alone.
All three peptides however induce migration of the human and mouse CD4+ T cells, but Defr1 has a higher optimal migration index of 9 compared with 5.5 for Y5C and Defr1-1cV and its peak is at a value of 10 ng/mL, tenfold more active than that of Defr1 Y5C and Defr1-1cV against human cells.
7, 8 Without any effect on cell viability, the number of migrating Langerhans cells cultured in a medium reconstituted with LRP-TSW was significantly lower than in a medium containing demineralized water (relative migration index = number of migrating Langerhans cells in medium with LRPTSW/number of migrating Langerhans cells in control medium = 0.5 ± 0.3 [mean of eight donors]).
