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Consequently these data are in line with the previous assumption that SFRP3 inhibits migration in melanoma cells by inhibiting Wnt5a signaling.
Interestingly, there was no decrease in migration after treatment with SFRP3 in cells where Wnt5a had been knocked down, suggesting that SFRP3 inhibits migration in melanoma cells via inhibition of Wnt5a (Figure 5A).
Based upon our data, R-Ras mediated migration in melanoma cells requires R-Ras binding directly to FLNa repeat 3. How this relates to the requirement for Rac and RLIP76 remains unclear.
As shown in Figure 5A, TGFβ strongly inhibited cell migration in melanoma cells.
Conversely, Src inhibition leads to decreased proliferation and migration in melanoma cell lines (Eustace et al, 2008, 2010).
However, LPA has also been found to inhibit migration in melanoma cells, and thereby act as a tumour suppressor [ 29].
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In conclusion, our results identify FAK as a key molecule at the crossroad between invasion and migration pathways in melanoma cells.
Time-lapse microscopy was used to track migration in the melanoma cells, as described previously (25).
Cdc42 is also implicated in amoeboid migration and invasion in melanoma cells.
MAP4K4 is involved in cell migration and invasion in melanoma, ovarian, breast and prostate cancers[ 31].
It is presently unclear how ARMS regulates migration and invasion in melanoma cells.
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