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Figure 2 presents the cell migration images of B16F10 cells in treated and untreated plates after fixing and staining, in which the maximum cell migration was observed in untreated control.
Wound closure or cell migration images were photographed when the scrape wound was introduced (0 hr) and at a designated time (48 hr) after wounding using an inverted microscope.
Photographed the cells migration images at 0, 36 h.
Migration images were analysed using Jelly 0.1 (Rami, unpublished) and exported as a data table.
To record cell migration, images of the wound were acquired at time zero and again 24 h later in an inverted microscope (Nikon Eclipse TS100) equipped with a digital camera (Nikon Ds Fi2, Nis elements F 4.00.00 software).
At 0 and 24 h after wounding, respectively, migration images were captured and documented at different time points using an inverted Olympus IX50 microscope with 10× objective lens and the Image-Pro Plus software (Media Cybernetics).
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They can be classified into three categories: application-centric migration, image-based migration and migration to a virtualized container.
In RTM with only primary energy or the P-wave mode in seismic data, the S-wave energy is regarded as noise for the migration image.
Figure 16b shows an across-arc seismic migration image below central Cascadia obtained via seismic receiver-function analyses (Abers et al. 2009).
When a pseudo-acoustic TTI (tilted transversely isotropic) coupled wave equation is used to implement reverse-time migration (RTM), shear wave energy is significantly included in the migration image.
For a noise-free migration image containing a complex events, the associated Hankel matrix of the data is a matrix of rank a, and the peak of the difference spectrum will exist at the ath point.
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