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Figure 3B shows the analysis from this experiment at the end time point (7 hs) indicating that whereas EGF+bFGF or FBS promote cell migration, cells in RA or DMEM are not able to migrate significantly.
Results are expressed as the migration cells in the experimental group relative to those in the control group.
To quantify migration, cells in the lower chamber were stained with calcein (BD), and the fluorescence was measured at Cytofluor4000 fluorescence reader (MTX Lab Sytems).
To evaluate the contribution of PI3K, mTOR, and ERK1/2 pathways to VVEC migration, cells in transwells were pre incubated with LY294002 (20 μM), wortmannin (100 nM), rapamycin (10 nM), or U0126 (10 μM) for 60 min.
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We sought to determine how TGF-β affects tumor cell migration in cells grown in different collagen concentrations and for different incubation times.
Importantly, the nanodisk lasers introduced here are small enough to allow unobstructed migration of cells in confined environments, which is demonstrated using NIH 3T3 cells as an example.
Proliferation or migration of cells in a tissue should be tightly regulated because the overactivation of such cellular functions can disrupt tissue homeostasis and even result in death.
The osmotic migration of cells in a solute gradient.
C5a is a potent chemokine regulating migration of cells in the innate immune system.
There is a modest increase in cell migration in cell lines overexpressing miR-30a compared to their controls.
Nonetheless, VT reduced trans-endothelial permeability and tumor cell migration in cell culture.
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