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Through this process, MCs gain higher invasive and migration capacities and promote peritoneal membrane fibrosis and functional changes [ 21, 22].
We also studied their actin cytoskeleton organisation, migration capacities and their regulation of PTEN and MEK1-2 activity.
We showed that RhoB induction in OVCAR-3A (open bars) mimic the actin cytoskeleton network organisation and density, inhibit their migration capacities and PTEN and MEK1/2 phosphorylation regulation observed in OVCAR-3LTE.
On the contrary, the inhibition of RhoB in OVCAR-3LTE (grey bars) drive us to observe the OVCAR-3A properties in actin cytoskeleton organisation and density, migration capacities and their regulation of PTEN and MEK1-2 activity we previously described.
We show in this study that RhoB induction in control cell lines (OVCAR-3A and WT) and its inhibition in OVCAR-3LTE is critical in ovarian cell lines we used for migration capacities and certainly for tumour aggressiveness together induced by trastuzumab treatment.
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Furthermore, mouse metastatic melanoma cells increased in size and elongation ratio, and displayed increased migration capacity and gelatinase secretion after exposure to acidic extracellular pH [26].
Moreover, Rac1 activity in the various cell types correlated with migration capacity, and introduction of active Rac1 into late HF1 cells increased their motility.
In line with these data, our knockdown experiments using RhoB specific siRNAs indicated a decreased migration capacity and a reduced organization into tubular structures in endothelial cells.
In this way, we have shown that this enzyme and its main product, SLex, confer on pancreatic carcinoma cells a major E-selectin adhesion, a high migration capacity and a high metastatic potential.
Consequences of overexpression were studied in terms of in vivo cell growth, gene expression, migration capacity and chemosensitivity.
They only investigated the expression of EMT markers but not the migration capacity and cytoskeletal redistribution of MCs.
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